The sequence of cell cycle events is governed by an array of proteins which regulates the essential process of cell division. The present study involved extraction of proteins from interphase and mitotic CHO cells, their purification and characterization by protein profiling and estimation of protein concentration. Protein profiling by SDS PAGE confirmed the presence of proteins unique to mitotic extracts with particular reference to a protein of molecular weight ~ 38 kDa . The study also focused on generating polyclonal antibodies against CHO cytosolic mitotic extract, testing their reactivity and employing them to mediate mitotic inhibition. Inhibition of mitosis was observed in a dose dependent manner. The results of the evaluation of homogeneity of cytosolic protein antigens tested by a combination of precipitation reactions in extracted protein solutions and also in situ whole cells by immunofluorescence confirmed that the reactivity of the antiserum was restricted to protein antigens of cancerous cell lines(CHO, Vero and HeLa) and that extracts from normal cells(lymphocytes) failed to react with the same.6609 Руб.
'Selective Stain For Mitotic Figures' is a text book mainly focusing on significance of identification of various phases of mitosis in histopathological tissue specimen of Oral squamous cell carcinoma and oral epithelial dysplasia. This book is highlighting various special stains used for staining of mitotic figures perticularly selectivity of crystal violet stain has been discussed. Various methods of counting of mitotic figures are also discussed in this book. At last reader will acquire knowledge of overview of Oral squamous cell carcinoma and Oral epithelial dysplasia, their etiological factors, pathogenisis and grading system.5735 Руб.
Antibody production is now a rapidly developing area where the uses of polyclonal antibodies are finding wide application. This book explains the antibody production starting from antigen preparation to anti-sera purification including protein dialysis and affinity chromatography. It also contains protocols for coating of anti-sera to solid phase and most buffers needed for polyclonal antibody production. It offers the basic principle of immunoassays.4763 Руб.
Scorpionism is a major health problem in under developed countries. No specific antiserum or any other antidote is available for the treatment of a patient suffering from scorpion sting and hence prepare of a potent antiserum against scorpion venom is an utmost requirement. This book describes the toxicological effects of Asian Black scorpion Heterometrus fastigiousus and the method to prepare species specific antiscorpion venom. It is hoped that results emerged will be useful in understanding the toxicological behaviour of scorpion venom and simultaneously antivenom raised will find a clinical application in neutralizing the toxic effects of scorpion venom.4579 Руб.
The effect of lasers of 3 wavelengths in the visible region - 476, 488 & 514 nm on mitotic & meiotic cell divisions, growth, yield & activity of specific enzymes were studied in two taxonomically diverse plant species - Allium cepa L. & Vicia faba. The effect of laser exposures was compared with the effect of two physical mutagens (gamma & UV radiations) and two chemical mutagens (Ethyl Methane Sulphonate and Hydroxyl amine; EMS & HA). The study indicated that lasers could be mutagenic causing aberrations in the mitotic & meiotic cell divisions & producing changes in the growth & yield of the plants. Lasers of higher wavelengths 488 & 514 nm caused aberrations in the early stages of mitotic cell division whereas lasers of lower wavelengths (476 nm) caused more aberrations in later stages. Laser exposure of 488 nm wavelength at power density 400 mW induced higher mitotic & meiotic aberrations & also induced higher pollen sterility than lasers of 476 & 514 nm. The frequency of mitotic aberrations induced by lasers was less than that caused by gamma-irradiation but comparable to that induced by EMS & HA. Lasers also stimulated growth & yield. Lasers cause more mutation than UV.7678 Руб.
In ELISA formats there are several parameters that are common and critical to assay performance and should be considered during the optimization process, including antibodies concentration, antigen concentration, incubation time of blocking, substrate solution and washing solution. Though immunoassays offer a specific, sensitive and rapid method to detect and quantify even trace amounts of allergens in foodstuffs, certain processes can denature, alter or destroy proteins so that they are no longer detectible by assay using antibodies. When using monoclonal antibodies, there is a great chance of obtaining false negatives in the detection. Polyclonal antibodies are recommended for ELISA techniques as the risk of obtaining false negatives is reduced as compared to monoclonal antibodies which are quite specific.4763 Руб.
Thrombotic thrombocytopenic purpura (TTP) is a life-threatening disease caused by a deficiency in ADAMTS13 which in the acquired form is provoked by autoantibodies against this protease. The main objective of this work was to study the pathophysiologic and prognostic value of anti-ADAMTS13 antibodies in patients with acquired TTP. The surveyed antibody profile revealed the presence of mainly IgG followed by IgA and IgM antibodies. IgG1-4 and IgA ADAMTS13-specific immune complex (ICs) were detected. IgG4 was the most prevalent IgG subclass, present either as free antibody or as ICs. IgG antibodies in combination with high inhibitor titers at disease presentation were associated with persistence of undetectable ADAMTS13 activity in clinical remission. TTP patients with high IgG4 and undetectable IgG1 levels were more prone to relapse than patients with low IgG4 and detectable IgG1 levels suggesting that IgG4 could be a useful biomarker for identification of patients at risk of disease recurrence. The comprehensive characterization of anti-ADAMTS13 antibodies as well as circulating ADAMTS13-specific ICs may contribute to a better understanding of the mechanisms leading to acquired TTP5056 Руб.
Plantago ovata (Isabgol), the important drug is grown principally for its seed husk valued for medicinal properties. Its seeds are used in catarrh, chronic constipation, amoebic and bacillary dysentery and diarrhea, intestinal flexes, discharge of mucus, affection of bladder, urethra and kidneys and functional derangement of digestive organ. At present there is no such book in the market that covers the investigatory aspects about cytological studies & effects of mutagens on this plant which can be used as a study material for scientists.This book provides the investigatory information about the mitosis & karyotype, meiosis, effect of physical & chemical mutagens in alone and combination treatments on mitotic index, mitotic abnormalities, meiotic abnormalities and chromosomal aberrations. This indispensable source book would be helpful for those who are working for the cytological studies of “imphagul”, mainly the scientists in Agriculture, Horticulture, Genetics & Plant Breeding and all others who are curious for the cytogenetic studies of this plant.The results mentioned here are the conclusions made by the authors which may vary in different geographic & climatic conditions.5735 Руб.
The control of an infectious viral disease as rabies is made easier by rapid and accurate diagnosis. Successful rabies prophylaxis is dependent upon the active immunization with vaccine along with passive administration of rabies virus neutralizing antibodies which together clear the virus before widespread infection of central nervous system occurs. The present study aimed at the development of monoclonal antibodies (MAbs) suitable for rabies virus antibody and antigen detection. The positive clones were selected on the basis of distinct reactivity by cell Enzyme linked immunosorbent assay and fluorescence in Indirect Fluorescent antibody test. The reactive clones were further titrated and employed for virus titration and virus neutralization. The neutralizing activity was evaluated using Fluorescence Activated Cell Sorter (FACS) technique. MAb clones showing distinct percent inhibition in the presence of positive serum were selected. MAb specific in detecting rabies antibodies and also found suitable for competitive ELISA to assess the antibody level in vaccinated subjects were selected.4763 Руб.
Human Herpes Virus Type 4 or Epstein Barr Virus is known to exert its oncogenic potential in altered immune states, HIV/AIDS being one of them. The HHV4 life cycle involves the continuous production of various latent and lytic proteins. In HIV infection antibodies to non HIV micro-organisms is erratic because the entire immune system is trying to combat the rapidly replicating HIV. HIV associated tumors appear in HIV clinical stage 3. Literature has not been able to demonstrate, in an HIV positive population, the association of HIV clinical staging with EBV antibodies and EBV infection clinical stages . Virologists and clinicians who are interested in the interaction of HIV and other oncogenic viruses will find this a valuable addition to their knowledge.4212 Руб.
Diagnosis of Antiphospholipid syndrome (APS) depends on laboratory findings, such as detection of Lupus Anticoagulant (LAC) and anticardiolipin antibodies (ACLa). Recurrent pregnancy loss (RPL) is the commonest pregnancy morbidity related to this condition. The syndrome is defined by the occurrence of venous and arterial thromboses and pregnancy morbidities, in the presence of anti phospholipid antibodies (aPA), namely LAC, ACLa and anti- ?2 glycoprotein I (?2GPI) antibodies.4763 Руб.
Intake of vitamin B12 for three consecutive weeks had no effect on the weight or body shape of the mice. Also, no toxic effects were observed during the experiment. Intake of vitamin B12 (0.8 mg / kg body weight) before irradiation improved the mortality rate, when compared to the untreated irradiated group and the best improvement was observed at day 14 after irradiation. Intake of vitamin B12 prior to irradiation improved the rate of mitotic index. The best recoveries in mitotic index rate were at 6 and 72 hr after irradiation. Treatment with vitamin B12 prior to irradiation increased the total number of polychromatic erythrocytes (PCEs) by 17 % and decreased the total incidence of normochromatic erythrocytes (NCEs) by 4 folds. Intake of vitamin B12 prior to irradiation revealed a highly significant decrease in the morphological deformation of sperm head and tail by 1.4 folds.5735 Руб.
In contrast to most other vertebrates,teleost fish exhibit an unparalleled capacity to generate stem cells in both the intact and the injured adult brain.My research is focused on the occurrence, the extent and the fate of aneuploid cells (genome-modified individual cells characterized by the loss or gain of whole chromosomes) generated in the teleostean brain. Aneuploidy arises through various chromosome segregation defects during the mitotic divisions of newly-generated cells in the adult brain. Aneuploidy is observed in ~80% of the newly-generated stem cells of the adult brain,but occurs at a very low rate in liver tissue, as shown by metaphase chromosome spreads and flow-cytometric DNA content analysis. The mitotic machinery of adult brain cells was examined for chromosome segregation defects which showed lagging chromosomes at metaphase and anaphase, as well as micronuclei and anaphase bridges,in the intact adult brain. In conclusion,my research demonstrates the evidence of the generation and maintenance of aneuploid cells in the adult fish brain that arise through chromosome missegregation which is a widespread phenomenon in the teleostean central nervous system.4763 Руб.
Monospecific antibodies are antibodies raised against a specific antigen used extensively in basic biomedical research, diagnosis and treatment of a disease such as infections and cancer. In the present study monospecific antibodies were produced against antigen B of the ABO blood group system to be used as a diagnostic marker for blood group B typing. The work was performed in the laboratory of Vaccinology and Molecular Immunology at the Center of Virology and Immunology, National University of Science and Technology. The research was supervised by Dr. Muhammad Ali A Shah and Co-supervised by Dr. Kashif Asghar; with the help of Dr. Taimoor Ashiq who was the internal member of the principal author, Bilal Zulfiqar. The work was fully guided by Dr. Isthtiaq Qadri, the Prinicipal of the Center.4763 Руб.
In malaria endemic regions, pregnant women and their newborns are at high risk of developing severe symptoms of malaria caused by Plasmodium falciparum. Placenta-sequestering malaria parasites involved in the pathogenesis express particular Variant Surface Antigens (VSAPAM) on the surface of infected erythrocytes (IEs). The mechanism underlying the association between PAM, placental pathology and poor pregnancy outcome remained poorly understood in human. Thus, the development of animal models to help understand these mechanisms is of utmost importance. The present study showed that pregnancy status and intensity of Plasmodium falciparum transmission can affect acquisition of Immunoglobulin G Subclass responses to VSAPAM in Cameroonian women. Observations from mice model suggest that levels of antibodies specific for recrudescence-type IEs are related to the protection of pregnant mice and their offsprings from poor outcomes. Alterations of cytokine levels may also strongly contribute to poor pregnancy outcome. The study of Plasmodium berghei malaria in pregnant, immune mice can be used to gain a better understanding on the mechanisms of malaria pathogenesis and immunity.5433 Руб.