Established cancer cell lines have and continue to contribute tremendously for many discoveries and are some of the important tools for studies involving drug and biomarker discovery. Cell culture techniques has seen a multifold change with advancements in the technology; the biocompatible material available as culture supports and with enhancing understanding of cell physiology and culture behaviors. Culturing cells as monolayers in the 2D system are extremely important for various applications, likewise, the 3D systems of cell cultures have thrown valuable insights which were not possible with the 2D systems. Culturing cells in a 3D model is considered to bring in a closer ‘in vivo’ effect to the cells and cells in such a system sure show marked changes compared to their 3D counterparts. Several matrices and supports are available for culturing cells in 3D. Agarose hydrogels offer us a useful combination of economy and ease of applications. We present here the uses of agarose...4615 Руб.
The sequence of cell cycle events is governed by an array of proteins which regulates the essential process of cell division. The present study involved extraction of proteins from interphase and mitotic CHO cells, their purification and characterization by protein profiling and estimation of protein concentration. Protein profiling by SDS PAGE confirmed the presence of proteins unique to mitotic extracts with particular reference to a protein of molecular weight ~ 38 kDa . The study also focused on generating polyclonal antibodies against CHO cytosolic mitotic extract, testing their reactivity and employing them to mediate mitotic inhibition. Inhibition of mitosis was observed in a dose dependent manner. The results of the evaluation of homogeneity of cytosolic protein antigens tested by a combination of precipitation reactions in extracted protein solutions and also in situ whole cells by immunofluorescence confirmed that the reactivity of the antiserum was restricted to protein...6240 Руб.
Cancer is a term that encompasses a complex group of more than 100 different types of diseases associated with uncontrolled cell division. Epigenetic events are alterations in gene expression without changes in DNA sequence and characteristic of heritability via cell division. Certainly, it is anticipated that aberrant changes bring about epigenetic control through chromatin modifying enzymes. This has an important bearing on future therapies for human diseases including cancer.4615 Руб.
What a tiny treasure in Salvia nutlets produces! Topics include pericarp structure of the nutlets, chemical - physicochemical properties of their hydrogels, and thermal stability of the hydrogels. Firstly nutlets of Salvia were grouped into 4 types in relation to the hydrogel productivity. Good co-relationship was observed between hydrogel content and pericarp thickness. Precise chemical analysis of the hydrogels from the nutlets of three species of Salvia, S. miltiorrhiza (SM), S. sclarea (SC) and S. viridis (SV), lead to the key finding that shows presence of composite structure of cellulose and hemicellulosic polysaccharides consisting of neutral galactan or arabinogalactan and acidic (1,4)-linked xylan highly substituted with glucuronic and 4-O-methylglucuronic acids (SC and SV) or glucuronic acid (SM). Co-operations of electrostatic repulsion, cross-linkage through calcium ions and hydrogen bond were important for formation of the hydrogel structure. Importance of water structure...5415 Руб.
In response to the rising demand for engineered tissues and organs, there have been great research interests to fabricate functional tissues in laboratories, and such products may revolutionize the current approaches for medicine and healthcare. However, to achieve this daunting task of mimicking functionality and complexity of native tissues, scaffolds have to be sufficiently vascularized with precise controls so that the resident cells can be perfused with oxygen and nutrients. The materials designed and cell culture techniques presented here demonstrate their capabilities to support endothelial cell morphogenesis into capillary-like structures. Specifically, poly(ethylene glycol)-based hydrogels were synthesized to promote and sustain therapeutic angiogenesis in hosts for wound healing and tissue engineering applications. By fine-tuning the biochemical and biophysical properties of gel matrices, micropatterning of biomolecules with photolithographic techniques, and co-culturing...7332 Руб.
Plant plays an important role as a source of many anticancer drugs with minimal side effects , for this purpose this study was designed to evaluate the cytotoxic effects of crude and pure extracts of Convolvulus arvensis plant on cancer and transformed cell lines in vitro .This book also included the effect of these extracts on mitotic index on cancer cell line and lymphocytes. The book was demonstrated that the pure extract significantly inhibits the treated cancer cell line proliferation and cause apoptosis. Different methods have been used for detection of apoptosis: light microscope, fluorescent microscope and DNA electrophoresis.4323 Руб.
Literature survey clearly indicates that fresh water prawns, despite being important member of food chain, good bio-indicator and having high economical value, have not been properly studied in relation to metal toxicity. Considering economical and good bio-indicator value of fresh water prawns, present work has been taken into account to evaluate behavioural, haematological and histopathological effects of mercuric chloride and nickel chloride on Macrobrachium lamarrei and Macrobrachium dayanum, prawn species, abundantly available in Lucknow. The research work incorporated in present thesis is represented in three sections. Section-I deals with bioassay experiments; Section-II deals with behavioural, haematological and histopathological effects of mercuric chloride while Section-III deals with behavioural haematological and histopathological effects of nickel chloride on fresh water prawns, Macrobrachium lamarrei and Macrobrachium dayanum, good bio-indicators and ...7250 Руб.
rtation results showed that the test extract recovers the blood parameter in curative and preventive study, while mechanistic study showed protection of RBC membrane when exposed to ruinous substances. Thus, present finding affirm assentation of this plant in folk medicine for the management of hemolytic anemia.Traditional oral report indicates that fruits of opuntia ficus indica are used in the treatment of anemia in India. It is cheaper and widely available plant. The claims reported in favour of opuntia ficus indica are not substained by modern scientific studies. For this purpose, the present study was carried out to validate effect of dehydrated water extract of opuntia ficus indica on experimentally-induced hemolytic anemia in rats. In mechanistic study, the protective actions of test extract on red blood cells against hypotonic solution, verapamil and calcium free medium was evaluated. Present book results showed that the test extract recovers the blood parameter in curative...4615 Руб.
Public relations is a distinctive management function which helps establish and maintain mutual lines of communication, understanding, acceptance and cooperation between an organization and its publics; involves the management of problems or issues; helps management to keep informed and responsive to public opinion; defines and emphasizes the responsibility of management to serve the public interest; helps management to keeps abreast of and effectively utilize change, serving as an early warning system to anticipate trends; and uses research and sound and ethical communication techniques as its principal tools. In this context this study tries to explore two-fold path. The first attempts to understand the way Public Relations and Corporate Communication departments are structured in organizations and the functions they perform in meeting the challenges of contingent environments. The second focuses on the management-induced constraints, if any, experienced by Public Relations or...4615 Руб.
Concrete is a composite construction material made primarily with aggregate, cement, and water. There are many formulations of concrete, which provide varied properties, and concrete is the most used man-made product in the world. Concrete is widely used for making architectural structures, foundations, brick/block walls, pavements, bridges, dams, pools/reservoirs, even boats etc. There is a general view that the size of coarse aggregate affects only the properties of fresh concrete and it is impact on hardened properties is insignificant. This book presents the impact of different sizes of coarse aggregate on fresh and hardened properties of concrete. While slump cone test evaluated the fresh concrete properties, compressive strength test determined the hardened state properties by using concrete mixtures that proportioned with three types of cements. Graded coarse aggregates with maximum aggregate size were selected for this experimental program for each cement. The results show...4615 Руб.
India is one of the four major countries in the world producing, exporting and importing oilseeds. Indian vegetable oil economy is the fourth largest in the world, next to the US, China and Brazil, accounting for about 14 per cent of the world’s oilseeds area and 8.5 per cent of the world’s Oilseeds production. India holds premier position in the world in terms of area and production and ranks first in the world in production of castor and safflower and it is second largest producer of groundnut and sesame and ranks third in linseed and rapeseeds, fifth and ninth in soyabean and sunflower respectively.In a country like India, the prices of agricultural commodities play an important role in the price structure of the country. The agricultural prices frequent fluctuate for various reasons. This book provides the importance of Oilseed crops in Indian economy and its significance on cultivation of production, profitability and farm business income of farmers at various farm levels...6240 Руб.
Thermoluminescence dosimetry (TLD) offers a very inexpensive and sensitive method of measuring atomic radiations. TLD are also used for radiation dose estimation in medical fields like in diagnostic radiology, radiation therapy and nuclear medicine. The research on thermoluminescence may be useful to obtain some information on the elementary excitation mechanism. Mechanoluminescence (ML) is a type of luminescence induced by any mechanical action on solids. In the recent past the intense mechanoluminescent materials have been found to have the potential for the stress sensor, fracture sensor, damage sensor etc. This book provides information on the thermoluminescence and mechanoluminescence, their mechanism, theories and experimental detail on the ZnS nanoparticles doped with Mn. A very cost effective mechanoluminescent (ML) device to study the glow curves by fracture method is explained. The effect on TL and ML intensity with respect to particle size was studied. This book is very...4615 Руб.
When compared to MTA cells, MTB-01 cells were susceptible to risedronateinduced apoptosis, had decreased ability to bind to risedronate-treated bone, and did not produce MMP-2 or MMP-9 proteases. MTA cells were less susceptible to risedronateinduced apoptosis and produced MMP-2. Additionally, adhesion of MTA cells to bone matrix was not diminished by risedronate treatment. Our results suggest that the nonresponsive nature of the MTA cell line may be due to MMP-2 production (possibly allowing ongoing destruction of risedronate-treated bone), continued adhesion to risedronate-treated bone matrix, and decreased susceptibility to risedronate-induced apoptosis.4830 Руб.
Marriage, in Indian social system, acquires an important place. The custom of marriage is largely governed by the fixed social customs and norms carried out from one to another generation. However, under the wave of present modernization, lot of changes has been observed in different dimensions of mate selection and marriage process. Although, a number of studies across the globe have documented the impact of education, employment and other proxies of modernisation on age at initiation of marital formation, the impact of changes in other dimensions of mate selection such as changes in marriage norms, laws pertaining to age at marriage, mate selection criteria, selection process, cost of marriage and many more on age at first marriage have not been discussed in detail. The views of unmarried individuals on mate selection and their transition to marriage under different circumstances also remained a neglected area for discussion. Using the first hand data, collected from the urban part...7250 Руб.
This monograph is about an artistic journey set off eighty years ago in the nascent Republic China. It charged into the “impossible” realm--nudity--in the grand Chinese tradition and turned it, if somewhat by accident, into the “possible”, unveiling perhaps the most censored body in the art world. The journey has since been progressing--at times in twists and turns--although the artist, Xu Beihong, who initiated it had died 14 years after the set-off--never had a chance to reap results--leaving behind a legacy largely misunderstood and undervalued. Xu Beihong, a Paris academy-trained Chinese painter at the turn of the 20th century, professed to reform a declining art at a time when China was seeking new political and cultural lights shed by the West. With a stern belief in the Western realism, Xu had brought home not just the realist techniques but also the classic iconography of the nude dated back to the Renaissance Venus. Xu had not offered us voluminous art works in this respect,...5130 Руб.
Some new 1,3,4 Thidiazoles were synthesized, derivatized, characterized by TLC, Melting point, their individual percentage yield were studied and selected compounds were characterized by IR, NMR spectroscopy and Mass spectrometry and their invitro anti cancer activity were tested on HT-29 cell line by employing MTT assay, the parent and few of the derivatives have shown significant IC50 activity.3382 Руб.